Methods: We compared pre-PP and post-PP lymphocyte subpopulations and plasma neopterin in 37, and cytokine plasma levels in 30, potential renal transplant recipients. Plasma neopterin and cytokines were measured by enzyme-linked immunosorbent assay kits, lymphocyte subsets were determined using four-color fluorescence flow cytometry.
Results: Lymphocyte subpopulation counts and ratios including CD45+/μL (P=0.005), CD3+/μL (P=0.02), CD4+DR+/μL (P=0.002), CD8+/μL (P=0.01), and CD8+DR+/μL (P=0.005) T cells; CD4+DR+/CD4+ (P=0.009) and CD8+DR+/CD8+ (P=0.0004) ratios; DR+ cells:μL (P=0.003); CD19+ B lymphocytes/μL (P=0.001); and plasma levels of neopterin (P<;0.0001), soluble interleukin-1 receptor antagonist (P<;0.0001), IL-8 (P=0.0001), and tumor necrosis factor-α (P=0.008) were significantly decreased after PP as compared with before PP. The results indicate a decrease of activated DR+, CD4+, and CD8+ T lymphocytes and B lymphocytes, and a decrease of monocyte and macrophage activation as a result of PP.
Conclusion: Based on these results, we conclude that PP not only removes antibodies from the plasma but, in addition, modulates T-lymphocyte activation and the inflammatory response by decreasing plasma proinflammatory cytokines (read more) Print this post
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